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referred to as retromutagenesis. The effectiveness of the antioxidant ascorbic acid on combating ROS accumulation was studied. Using Saccharomyces cerevisiae as a model organism, we investigated the replicational mutation rates and adaptive mutation frequencies of ogg1Δ cell lines with ascorbic acid ranging in concentration from 0 to 100 mM. The ogg1Δ cell line was used for its inability to repair oxidative DNA damage. The presence of ascorbic acid had no discernible effect on the generation of

plays a role in DNA damage repair of guanine and affects the prevalence of mutations in cells in response to oxidative stress. This study looks into the differences between adaptive and replicative mutagenesis induced by oxidative DNA damage in order to determine underlying mechanisms that may have taken place. In Saccharomyces cerevisiae, the occurrence of adaptive mutants after induced DNA damage in wild-type (WT) cells increased by 6.52-fold and in ogg1 cells by 1.33-fold. The replicative

accordance with the manufacturer’s designed or intended purpose.2.0 Hand and Power Tools (WAC 296-155, Part G)Employees will use proper tools suitable to the job being done. Only tools in good repair may be kept or used on the premises or on the job. Employee-owned tools must meet all safety requirements, whenever used for work. Guards must be in place when so designed. 2.1 Hand Tools Use the proper tool for the job. When possible, purchase tools with ergonomic features. The following guidelines apply to

). The title compound was synthesized in a three step reaction starting from o-xylene and propionic acid. Ferric chloride was used to catalyze the electrophilic aromatic substitution to form a ketone intermediate, followed by a simple bromination and addition of methylamine to yield the crude product. Product was purified by recrystallization in diethyl ether. The final compound was characterized by 1H NMR, 13C NMR, UV-Vis, GC-MS, FT-IR, and melting point. 3:10 pm - Oxidative DNA Damage in

confirm the shifts and size of the peaks to indicate nanocrystals increasing in length. Therefore, transmission electron microscopy must be run in order to obtain further morphological and crystallographic information about the nanocrystal products. 3:00-3:25 pm Nic Celebrado, Senior Capstone Seminar The importance of DNA and its preservation can be noted due to the presence of many repair mechanisms developed to mitigate the potential harm DNA damage and its subsequent mutations can cause to the

only.University CalendarIf you would like your event posted on the PLU University Event Calendar, go to www.plu.edu/calendar and register your event using 25Live.Die-Cut PolicyThis machine is hand operated and is used to produce multiple copies of precision cut letters and shapes using commercially-produced dies. This machine is primarily for student club/organization use. Please email engage@plu.edu to set up a time to use the machine. Any damage to the machine caused by negligence will be the responsibility

2017 AwardsCongratulations to the Overall Winners of the 2017 South Sound Regional Science & Engineering Fair! The top three overall winners in the grades 9-12 category earned a Grand Award of an all expenses paid trip to the 2017 Intel ISEF May 14-19 in Los Angeles, CA. 1st Place: Ashlynn Gallagher, W.F. West High School: A Novel Approach to Understanding Cancer: Using Antitumor Agents In The G2/M DNA Damage Checkpoint In The P53 Gene On The Early Development of Zebrafish Embryos 2nd Place

steps provided below.  Laptop users will want to make a note of this, as once the installation has started you will need to wait until the updates are done to unplug and put away your computer.  Turning off the machine before the updates have completed can cause damage to the system files, resulting in system instability. Windows XP: Windows XP users will be prompted with the window to the right when they go to shut down and updates are ready to install.  To avoid installing the updates now, simply

Management Planning Worksheet Student Activity/Club WaiverVehicle Rental and InsuranceIn accordance with insurance law, PLU’s auto physical damage insurance policy does not provide coverage for use of private vehicles driven for university business. When renting a vehicle in the United States DO NOT purchase rental company auto insurance. When renting outside of the United States, you MUST purchase rental company auto insurance. Information on PLU’s vehicle rental program can be found on the Facilities

. Transcriptional mutagenesis as a mode for adaptive mutagenesis, and its involvement in cancers Aria E. Manning, Senior Capstone Seminar The mechanism and implications of transcriptional mutagenesis are of interest to many scientists. DNA integrity is of great important because DNA provides the code for the creation of proteins. DNA lesions can occur spontaneously or due to environmental pressures such as UV radiation. Cells have specialized repair systems to repair these lesions before replication or